• Q53 CecB (Cecropin B) peptide

Q53 CecB (Cecropin B) peptide

Not For Human Use, Lab Use Only.

Cat.#: 318819

Size:
Optional Service: TFA RemovalWhat's this?

Special Price 437.8 USD

Availability: 5 weeks
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Q53 CecB (Cecropin B) peptide
  • Documents
  • Sequence
    H-AGWLRKLGKKIQRIGQHTRDASIQVLGIAQQAANVAATAR-NH2
  • Three letter code
    H-Ala-Gly-Trp-Leu-Arg-Lys-Leu-Gly-Lys-Lys-Ile-Gln-Arg-Ile-Gly-Gln-His-Thr-Arg-Asp-Ala-Ser-Ile-Gln-Val-Leu-Gly-Ile-Ala-Gln-Gln-Ala-Ala-Asn-Val-Ala-Ala-Thr-Ala-Arg-NH2
  • Length (aa)
    40
  • Peptide Purity (HPLC)
    95.2%
  • Molecular Formula
    C187H321N65O51
  • Molecular Weight
    4295.93
  • Source
    Synthetic
  • Additional Information
    These cecropin B isoforms differ in a single amino acid substitution within the active portion of the peptide, so that the glutamic acid of the E53 CecB variant is replaced by a glutamine in the Q53 CecB isoform. Both peptides showed a high antimicrobial and membranolytic activity against P. aeruginosa, with Q53 CecB displaying greater activity compared with the E53 CecB isoform. Biophysical analyses, live-cell NMR, and moleculardynamic-simulation studies indicated that both peptides might act as membrane-interacting elements, which can disrupt outermembrane organization, facilitating their translocation toward the inner membrane of the bacterial cell. Our data also suggest that the amino acid variation of the Q53 CecB isoform represents a critical factor in stabilizing the hydrophobic segment that interacts with the bacterial membrane, determining the highest antimicrobial activity of the whole peptide. Its high stability to pH and temperature variations, tolerance to high salt concentrations, and low toxicity against human cells make Q53 CecB a promising candidate in the development of CecB-derived compounds against P. aeruginosa.
  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
  • References
    • Romoli O, Mukherjee S, Mohid SA, et al. Enhanced Silkworm Cecropin B Antimicrobial Activity against Pseudomonas aeruginosa from Single Amino Acid Variation. ACS Infect Dis. 2019;5(7):1200-1213. doi:10.1021/acsinfecdis.9b00042
  • About TFA salt

    Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

    TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

    TFA Removal Service is recommended for:

    • Peptides that will be used in cellular assays
    • Peptides that will be used as APIs or in manufactured products
    • For hydrophilic peptides containing numerous basic residues

  • Molar Concentration Calculator

  • Dilution Calculator

  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight

Peptide Property

  • Analysed Sequence:H-AGWLRKLGKKIQRIGQHTRDASIQVLGIAQQAANVAATAR-Amidation
  • Chemical Formula:C187H321N65O51
  • Sequence length:40
  • Extinction coefficient:5690 M-1cm-1
  • GRAVY:-0.25
  • Mw average:4295.93
  • Theoretical pI:12.41
  • Data Source:Peptide Property Calculator

GRAVY = grand average of hydropathy

X: Hydrophobic uncharged residues, like F I L M V W A and P

X: Basic residues, like R K H

X: Acidic residues, like D E

X: Polar uncharged residues, like G S T C N Q and Y

Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"