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Product Name
PYGL Polyclonal Antibody
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Description
Polyclonal antibody to PYGL
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PYGL antibody; GSD6 antibody; glycogen phosphorylase L antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PYGL (NP_002854.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - PYGL Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PYGL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - PYGL Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using PYGL antibody . Blue: DAPI for nuclear staining.
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Background
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"