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Product Name
PYGL antibody
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Description
PYGL Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, HeLa cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 97-105 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
GSD6 antibody; phosphorylase antibody; glycogen antibody; liver antibody; PYGL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002863). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114360(PYGL antibody) at dilution of 1:500
IP Result of anti-PYGL (IP:Catalog No:114360, 4ug; Detection:Catalog No:114360 1:500) with HepG2 cells lysate 2400ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:114360(PYGL Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PYGL belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). This antibody is specific to PYGL. It has no cross reaction to PYGM and PYGB.
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