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Product Name
PYGB Polyclonal Antibody
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Description
Polyclonal antibody to PYGB
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
PYGB antibody; GPBB antibody; glycogen phosphorylase B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:20 - 1:100
IP 1:50 - 1:100 -
Validations
Western blot - PYGB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PYGB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Western blot - PYGB Polyclonal Antibody
Western blot analysis of extracts of various cells, using PYGB antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5min.
Immunofluorescence - PYGB Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using PYGB antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - PYGB Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PYGB antibody . Western blot was performed from the immunoprecipitate using PYGB antibody at a dilition of 1:1000.
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Background
Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"