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Product Name
PYCR2 Polyclonal Antibody
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Description
Polyclonal antibody to PYCR2
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
PYCR2 antibody; HLD10 antibody; P5CR2 antibody; pyrroline-5-carboxylate reductase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human PYCR2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - PYCR2 Polyclonal Antibody
Western blot analysis of extracts of K-562 cells, using PYCR2 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. Involved in cellular response to oxidative stress.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"