PYCR1 antibody

Cat.#: 114354

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Product Information

  • Product Name
    PYCR1 antibody
  • Documents
  • Description
    PYCR1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, COLO 320 cells. Observed molecular weight by Western-blot: 33 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    P5C antibody; P5C reductase 1 antibody; P5CR antibody; P5CR 1 antibody; PIG45 antibody; PP222 antibody; PYCR antibody; PYCR1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PYCR1 recombinant protein (Accession Number: BC022244). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IF: 1:50-1:500

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114354(PYCR1 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114354(PYCR1 antibody) at dilution of 1:1000

    IP Result of anti-PYCR1 (IP:Catalog No:114354, 4ug; Detection:Catalog No:114354 1:1000) with HeLa cells lysate 3200ug.

    IP Result of anti-PYCR1 (IP:Catalog No:114354, 4ug; Detection:Catalog No:114354 1:1000) with HeLa cells lysate 3200ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:114354(PYCR1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:114354(PYCR1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"