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Product Name
PVRL4 antibody
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Description
PVRL4 Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IF detected in NIH/3T3 cells. Positive WB detected in HeLa cells, A2780 cells, MCF7 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 43 kDa, 66 kDa
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Tested applications
ELISA, WB, IF, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Ig superfamily receptor LNIR antibody; LNIR antibody; nectin 4 antibody; poliovirus receptor related 4 antibody; PRR4 antibody; PVRL4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PVRL4 recombinant protein (Accession Number: NM_030916). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114346(nectin 4 antibody) at dilution of 1:1000
Immunofluorescent analysis of NIH/3T3 cells using Catalog No:114346(nectin 4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 MCF-7 cells were stained with 0.2ug nectin 4 antibody (Catalog No:114346, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma.
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