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Product Name
PUS10 Polyclonal Antibody
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Description
Polyclonal antibody to PUS10
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Tested applications
WB
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Species reactivity
Mouse, Rat
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Alternative names
PUS10 antibody; CCDC139 antibody; DOBI antibody; pseudouridylate synthase 10 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - PUS10 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PUS10 antibody at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"