PUMA antibody

PUMA antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

PUMA Rabbit Polyclonal antibody. Positive WB detected in A549 cells, HepG2 cells. Positive IP detected in mouse heart tissue. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 18-21 kDa

ELISA, WB, IP, IHC

Human,Mouse,Rat; other species not tested.

BBC3 antibody; JFY 1 antibody; JFY1 antibody; PUMA antibody

Rabbit IgG

This antibody was obtained by immunization of Peptide (Accession Number: NM_014417). Purification method: Antigen affinity purified.

Polyclonal

0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

PUMA, also named as JFY-1 and BBC3, belongs to the Bcl-2 family. It is a critical mediator of p53-dependent and -independent apoptosis induced by a wide variety of stimuli. It serves as a proximal signaling molecule whose expression is regulated by transcription factors in response to these stimuli. PUMA transduces death signals primarily to the mitochondria, where it acts indirectly on the Bcl-2 family members Bax and/or Bak by relieving the inhibition imposed by antiapoptotic members. It directly binds and antagonizes all known antiapoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. PUMA ablation or inhibition leads to apoptosis deficiency underlying increased risks for cancer development and therapeutic resistance. It is a general sensor of cell death stimuli and a promising drug target for cancer therapy and tissue damage. It is essential mediator of p53-dependent and p53-independent apoptosis. (PMID: 19641508 )The antibody is specific to PUMA.

• Liu CJ, Zhang XL, Luo DY. Exogenous p53 upregulated modulator of apoptosis (PUMA) decreases growth of lung cancer A549 cells. Asian Pacific journal of cancer prevention : APJCP. 16(2):741-6. 2015.
• Li H, Liu Q, Wang Z. The oncoprotein HBXIP modulates the feedback loop of MDM2/p53 to enhance the growth of breast cancer. The Journal of biological chemistry. 290(37):22649-61. 2015.