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PUF60 antibody

Cat.#: 114336

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Product Information

  • Product Name
    PUF60 antibody
  • Documents
  • Description
    PUF60 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, mouse testis tissue, rat testis tissue. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 60 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FBP interacting repressor antibody; FIR antibody; FLJ31379 antibody; PUF60 antibody; Ro binding protein 1 antibody; RoBP1 antibody; RoBPI antibody; Siah binding protein 1 antibody; Siah BP1 antibody; SIAHBP1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PUF60 recombinant protein (Accession Number: NM_014281). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114336(PUF60 antibody) at dilution of 1:800

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114336(PUF60 antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114336(PUF60 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114336(PUF60 antibody) at dilution of 1:100 (under 10x lens)

  • Background
    Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiaed by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no reperssion activity in xeroderma pigmentosum complementation group B (XPB) cells.
  • References
    • Atanassov BS, Dent SY. USP22 regulates cell proliferation by deubiquitinating the transcriptional regulator FBP1. EMBO reports. 12(9):924-30. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"