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Product Name
PTRF antibody
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Description
PTRF Rabbit Polyclonal antibody. Positive IHC detected in human pancreas tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse lung tissue. Positive WB detected in A549 cells, HT-1080 cells, human heart tissue, mouse lung tissue, mouse skin tissue, rat heart tissue. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cavin 1 antibody; FKSG13 antibody; PTRF antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PTRF recombinant protein (Accession Number: BC008849). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:114332(PTRF antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:114332(PTRF antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PTRF antibody Catalog No:114332 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-PTRF (IP:Catalog No:114332, 3ug; Detection:Catalog No:114332 1:800) with mouse lung tissue lysate 5160ug.
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Background
Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transciption of RNA Pol I, which involves pausing of transcription by TTF1, and dissocaition of the transcription complex, release the complex from the template.
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References
- Inder KL, Ruelcke JE, Petelin L. Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation. Journal of extracellular vesicles. 3:. 2014.
- Regazzetti C, Dumas K, Lacas-Gervais S. Hypoxia inhibits Cavin-1 and Cavin-2 expression and down-regulates caveolae in adipocytes. Endocrinology. 156(3):789-801. 2015.
- Nassar ZD, Hill MM, Parton RG, Francois M, Parat MO. Non-caveolar caveolin-1 expression in prostate cancer cells promotes lymphangiogenesis. Oncoscience. 2(7):635-45. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"