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PTPRS antibody

Cat.#: 114330

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Product Information

  • Product Name
    PTPRS antibody
  • Documents
  • Description
    PTPRS Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in HeLa cells, mouse brain tissue. Positive IHC detected in human ovary tumor tissue, human ovary tissue, human pancreas tissue, rat brain tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 75-100 kDa
  • Tested applications
    ELISA, WB, IHC, IP, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    PTPRS antibody; PTPSIGMA antibody; R PTP S antibody; R PTP sigma antibody; rPTPσ antibody
  • Immunogen
    Human rPTPσ Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PTPRS recombinant protein (Accession Number: BC029496). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114330(rPTPσ antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114330(rPTPσ antibody) at dilution of 1:1000

    IP Result of anti-rPTPσ (IP:Catalog No:114330, 5ug; Detection:Catalog No:114330 1:400) with mouse brain tissue lysate 6500ug.

    IP Result of anti-rPTPσ (IP:Catalog No:114330, 5ug; Detection:Catalog No:114330 1:400) with mouse brain tissue lysate 6500ug.

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114330(rPTPσ antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114330(rPTPσ antibody) at dilution of 1:100 (under 10x lens)

    1X10^6 HeLa cells were stained with 0.2ug rPTPσ antibody (Catalog No:114330, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug rPTPσ antibody (Catalog No:114330, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Type IIa receptor protein tyrosine phosphatases (rPTPσ) are cell surface receptors important for nervous system development, function, and repair.The expression ofrPTPσ has previously been reported in b-cells and other target organs for insulin although the probes chosen did not permit to distinguish between the splice variants.Proteolytic processing near the transmembrane domain generates an extracellular N-terminal E-domain of 130 kDa and a C-terminal P-domain of approximately 85 kDa of rPTPσ,and the short splice variants rPTPσ 3 and 4contain an E-domain of 95 kDa(PMID:16552719). rPTPσ expression was observed in tissue lysates of the adult mouse sensory-motor cortex and thoracic spinal cord (T8-T10) as a 75–80kDa immunoreactive band(PMID:19780196).
  • References
    • Liu H, Xu H, Yu T, Yao J, Zhao C, Yin ZQ. Expression of perineuronal nets, parvalbumin and protein tyrosine phosphatase σ in the rat visual cortex during development and after BFD. Current eye research. 38(10):1083-94. 2013.
    • Gomez AM, Froemke RC, Burden SJ. Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4. eLife. 3:e04287. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"