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Product Name
PTPN22 Polyclonal Antibody
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Description
Polyclonal antibody to PTPN22
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PTPN22 antibody; LYP antibody; LYP1 antibody; LYP2 antibody; PEP antibody; PTPN22.5 antibody; PTPN22.6 antibody; PTPN8 antibody; tyrosine-protein phosphatase non-receptor type 22 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PTPN22 (NP_036543.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - PTPN22 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PTPN22 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - PTPN22 Polyclonal Antibody
Immunofluorescence analysis of C6 cells using PTPN22 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - PTPN22 Polyclonal Antibody
Immunofluorescence analysis of L-929 cells using PTPN22 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - PTPN22 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using PTPN22 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2. Positively regulates toll-like receptor (TLR)-induced type 1 interferon production. Promotes host antiviral responses mediated by type 1 interferon (By similarity). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"