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Product Name
PTH Polyclonal Antibody
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Description
Polyclonal antibody to PTH
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
PTH antibody; PTH1 antibody; parathyroid hormone antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-115 of human PTH (NP_000306.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - PTH Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PTH antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
Immunofluorescence - PTH Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PTH antibody . Blue: DAPI for nuclear staining.
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Background
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"