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Product Name
PTGER2 Polyclonal Antibody
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Description
Polyclonal antibody to PTGER2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PTGER2 antibody; EP2 antibody; prostaglandin E receptor 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human PTGER2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:100 - 1:200 -
Validations
Western blot - PTGER2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PTGER2 antibody at 1:200 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - PTGER2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using PTGER2 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PTGER2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using PTGER2 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - PTGER2 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using PTGER2 antibody .
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Background
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"