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Product Name
PSRC1 Polyclonal Antibody
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Description
Polyclonal antibody to PSRC1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PSRC1 antibody; DDA3 antibody; FP3214 antibody; proline and serine rich coiled-coil 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human PSRC1 (NP_001005290.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - PSRC1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PSRC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - PSRC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using PSRC1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PSRC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using PSRC1 antibody at dilution of 1:100 (40x lens).
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Background
Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate, and for normal rate of chromosomal segregation during anaphase. Plays a role in the regulation of mitotic spindle dynamics. Increases the rate of turnover of microtubules on metaphase spindles, and contributes to the generation of normal tension across sister kinetochores. Recruits KIF2A and ANKRD53 to the mitotic spindle and spindle poles. May participate in p53/TP53-regulated growth suppression.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"