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Product Name
PSMG1 Polyclonal Antibody
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Description
Polyclonal antibody to PSMG1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
PSMG1 antibody; C21LRP antibody; DSCR2 antibody; LRPC21 antibody; PAC-1 antibody; PAC1 antibody; proteasome assembly chaperone 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human PSMG1 (NP_003711.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - PSMG1 Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using PSMG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 5s.
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Background
Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"