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Product Name
PSMF1 Polyclonal Antibody
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Description
Polyclonal antibody to PSMF1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PSMF1 antibody; PI31 antibody; proteasome inhibitor subunit 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human PSMF1 (NP_006805.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - PSMF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PSMF1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - PSMF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using PSMF1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PSMF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using PSMF1 antibody at dilution of 1:50 (40x lens).
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Background
Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"