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Product Name
PSME4 antibody
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Description
PSME4 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, Hela cells. Positive WB detected in HEK-293 cells, HepG2 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 140kd
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ21864 antibody; KIAA0077 antibody; PA200 antibody; Proteasome activator PA200 antibody; PSME4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: BC113668). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114249(PSME4 antibody) at dilution of 1:400
IP Result of anti-PSME4 (IP:Catalog No:114249, 3ug; Detection:Catalog No:114249 1:500) with HEK-293 cells lysate 1760ug.
Immunofluorescent analysis of HepG2 cells, using PSME4 antibody Catalog No:114249 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa.
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