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Product Name
PSMD9 Polyclonal Antibody
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Description
Polyclonal antibody to PSMD9
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PSMD9 antibody; Rpn4 antibody; p27 antibody; proteasome 26S subunit, non-ATPase 9 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human PSMD9 (NP_002804.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - PSMD9 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PSMD9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .
Immunohistochemistry - PSMD9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using PSMD9 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - PSMD9 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using PSMD9 antibody . Blue: DAPI for nuclear staining.
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Background
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"