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Product Name
PSMD8 Polyclonal Antibody
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Description
Polyclonal antibody to PSMD8
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
PSMD8 antibody; HEL-S-91n antibody; HIP6 antibody; HYPF antibody; Nin1p antibody; Rpn12 antibody; S14 antibody; p31 antibody; proteasome 26S subunit, non-ATPase 8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 101-350 of human PSMD8 (NP_002803.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - PSMD8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PSMD8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - PSMD8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using PSMD8 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - PSMD8 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PSMD8 antibody .
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Background
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"