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PSMD2 antibody

Cat.#: 114398

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Product Information

  • Product Name
    PSMD2 antibody
  • Documents
  • Description
    PSMD2 Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in human heart tissue, HeLa cells, K-562 cells, mouse skeletal muscle tissue, rat heart tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 97 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    26S proteasome subunit p97 antibody; P97 antibody; Protein 55.11 antibody; PSMD2 antibody; Rpn1 antibody; TRAP2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PSMD2 recombinant protein (Accession Number: NM_002808). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114398(PSMD2 antibody) at dilution of 1:500

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114398(PSMD2 antibody) at dilution of 1:500

    IP Result of anti-PSMD2 (IP:Catalog No:114398, 3ug; Detection:Catalog No:114398 1:500) with K-562 cells lysate 3600ug.

    IP Result of anti-PSMD2 (IP:Catalog No:114398, 3ug; Detection:Catalog No:114398 1:500) with K-562 cells lysate 3600ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:114398(PSMD2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:114398(PSMD2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Tumor necrosis factor type 1 receptor-associated protein 2 (TRAP2), encoded by PSMD2 gene, is a non-ATPase regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. TRAP2 may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor.
  • References
    • Nijhawan D, Zack TI, Ren Y. Cancer vulnerabilities unveiled by genomic loss. Cell. 150(4):842-54. 2012.
    • Sparks A, Dayal S, Das J, Robertson P, Menendez S, Saville MK. The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a. Oncogene. 33(38):4685-96. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"