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Product Name
PSMC3 Polyclonal Antibody
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Description
Polyclonal antibody to PSMC3
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PSMC3 antibody; TBP1 antibody; proteasome 26S subunit, ATPase 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PSMC3 (NP_002799.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - PSMC3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PSMC3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - PSMC3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat intestine using PSMC3 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - PSMC3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using PSMC3 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PSMC3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using PSMC3 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - PSMC3 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using PSMC3 antibody .
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Background
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC3 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"