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Product Name
PSMC2 antibody
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Description
PSMC2 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human ovary tumor tissue, human breast cancer tissue. Positive WB detected in human adrenal gland tissue, mouse liver tissue, mouse spleen tissue. Positive IP detected in mouse spleen tissue. Observed molecular weight by Western-blot: 49kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MSS1 antibody; Nbla10058 antibody; Protein MSS1 antibody; PSMC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMC2 recombinant protein (Accession Number: NM_002803). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human adrenal gland tissue were subjected to SDS PAGE followed by western blot with Catalog No:114389(PSMC2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114389(PSMC2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114389(PSMC2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using PSMC2 antibody Catalog No:114389 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-PSMC2 (IP:Catalog No:114389, 4ug; Detection:Catalog No:114389 1:500) with mouse spleen tissue lysate 4800ug.
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Background
PSMC2 is also named as MSS1 and belongs to the AAA ATPase family. PSMC2 is 1 of 6 putative ATPases contained within the regulatory complex. PSMC2 is first identified as a possible cellular factor that cooperates with the human immunodeficiency virus-1 (HIV-1) protein Tat, a potent activator of virus gene expression. The PSMC2 gene product works in association with the small subunit of mitoribosomes and seems to play some part in mitochondrial translation(PMID:8392589).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"