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Product Name
PSMB5 antibody
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Description
PSMB5 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human hepat℃irrhosis tissue. Positive WB detected in L02 cells, HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 22kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
20S proteasome antibody; LMPX antibody; Macropain epsilon chain antibody; MB1 antibody; Proteasome chain 6 antibody; Proteasome epsilon chain antibody; Proteasome subunit beta type 5 antibody; Proteasome subunit MB1 antibody; Proteasome subunit X antibody; PSMB5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMB5 recombinant protein (Accession Number: NM_002797). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:114385(PSMB5 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114385(PSMB5 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114385(PSMB5 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114385(PSMB5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of PSMB5 expression resulted in restoration of bortezomib sensitivity in resistant cells(PMID:18565852).It has 2 isoforms produced by alternative splicing.
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References
- Nijhawan D, Zack TI, Ren Y. Cancer vulnerabilities unveiled by genomic loss. Cell. 150(4):842-54. 2012.
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