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Product Name
PSMB2 antibody
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Description
PSMB2 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HEK-293 cells, HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 23kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
20S proteasome antibody; HC7 I antibody; Macropain subunit C7 I antibody; Proteasome component C7 I antibody; Proteasome subunit beta type 2 antibody; PSMB2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMB2 recombinant protein (Accession Number: BC000268). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114382(PSMB2 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114382(PSMB2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114382(PSMB2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114382(PSMB2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PSMB2(proteasome subunit beta type-2) is also named as macropain subunit C7-I, proteasome component C7-I, multicatalytic endopeptidase complex subunit C7-I and belongs to the peptidase T1B family. It is involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD(vitamin A depletion) brain regions, which may underlie the VAD-related psychosis behavior(PMID:21190828).
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