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Product Name
PSMB1 Polyclonal Antibody
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Description
Polyclonal antibody to PSMB1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PSMB1 antibody; HC5 antibody; PMSB1 antibody; PSC5 antibody; proteasome subunit beta 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-241 of human PSMB1 (NP_002784.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - PSMB1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PSMB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - PSMB1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using PSMB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PSMB1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using PSMB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PSMB1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate cancer using PSMB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PSMB1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophageal cancer using PSMB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PSMB1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using PSMB1 antibody at dilution of 1:100 (40x lens).
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Background
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"