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Product Name
PSMB1 antibody
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Description
PSMB1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human brain tissue. Positive IF detected in Hela cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 25kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
20S proteasome antibody; FLJ25321 antibody; HC5 antibody; KIAA1838 antibody; Macropain subunit C5 antibody; PMSB1 antibody; Proteasome component C5 antibody; Proteasome gamma chain antibody; Proteasome subunit beta type 1 antibody; PSC5 antibody; PSMB1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMB1 recombinant protein (Accession Number: NM_002793). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114379(PSMB1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114379(PSMB1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using PSMB1 antibody Catalog No:114379 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.
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References
- Ho J, Kong JW, Choong LY. Novel breast cancer metastasis-associated proteins. Journal of proteome research. 8(2):583-94. 2009.
- Wang J, Xu L, Yun X. Proteomic analysis reveals that proteasome subunit beta 6 is involved in hypoxia-induced pulmonary vascular remodeling in rats. PloS one. 8(7):e67942. 2013.
- Chen HX, Tang SP, Gao FT. Fibrosis, adipogenesis, and muscle atrophy in congenital muscular torticollis. Medicine. 93(23):e138. 2014.
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