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Product Name
PSMA1 antibody
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Description
PSMA1 Rabbit Polyclonal antibody. Positive WB detected in COLO 320 cells, HeLa cells, mouse kidney tissue, PC-3 cells. Positive IF detected in PC-3 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 29-33 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
20S proteasome antibody; 30 kDa prosomal protein antibody; HC2 antibody; Macropain subunit C2 antibody; PROS 30 antibody; PROS30 antibody; Proteasome component C2 antibody; Proteasome nu chain antibody; PSC2 antibody; PSMA1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMA1 recombinant protein (Accession Number: NM_002786). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:114372(PSMA1 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114372(PSMA1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:114372(PSMA1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PSMA1(Proteasome subunit alpha type-1) is also named as HC2, NU, PROS30, PSC2 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It is induced in breast cancer tissue and up-regulated in liver tumor tissues(PMID:17127214;16317774;17004105).
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