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Product Name
PSMA/GCPII antibody
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Description
PSMA/GCPII Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, mouse kidney tissue, PC-3 cells, rat kidney tissue. Positive IHC detected in human prostate cancer tissue, human colon cancer tissue, human gliomas tissue. Observed molecular weight by Western-blot: 100 kDa, 120 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
FGCP antibody; Folate hydrolase 1 antibody; FOLH antibody; FOLH1 antibody; GCP2 antibody; GCPII antibody; Glutamate carboxypeptidase 2 antibody; Glutamate carboxypeptidase II antibody; mGCP antibody; NAALAD1 antibody; NAALAdase antibody; NAALADase I antibody; PSM antibody; PSMA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMA/GCPII recombinant protein (Accession Number: NM_001014986). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114371(FOLH1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114371(FOLH1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114371(FOLH1 antibody) at dilution of 1:100 (under 40x lens)
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Background
PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.
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