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Product Name
PSEN2 Polyclonal Antibody
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Description
Polyclonal antibody to PSEN2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PSEN2 antibody; AD3L antibody; AD4 antibody; CMD1V antibody; PS2 antibody; STM2 antibody; presenilin-2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human PSEN2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - PSEN2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PSEN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunohistochemistry - PSEN2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using PSEN2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PSEN2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using PSEN2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - PSEN2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PSEN2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis. Is a regulator of mitochondrion-endoplasmic reticulum membrane tethering and modulates calcium ions shuttling between ER and mitochondria.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"