-
Product Name
PSAP antibody
- Documents
-
Description
PSAP Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse liver tissue, HepG2 cells, human liver tissue. Observed molecular weight by Western-blot: 65 kDa, 70 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
A1 activator antibody; Cerebroside sulfate activator antibody; Co beta glucosidase antibody; Component C antibody; CSAct antibody; Dispersin antibody; FLJ00245 antibody; GLBA antibody; Glucosylceramidase activator antibody; Proactivator polypeptide antibody; prosaposin antibody; Protein C antibody; PSAP antibody; SAP 1 antibody; SAP 2 Saposin D antibody; SAP1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PSAP recombinant protein (Accession Number: NM_002778). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114289(PSAP antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114289(PSAP antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114289(PSAP antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:114289(PSAP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
The PSAP gene encodes prosaposin, a precursor of fourl small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin A. Read more about this antibody on the blog:http://blog.ptglab.com/index.php/psap-antibody-uncovers-potential-role-of-prosaposin-in-cancer-survival/
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"