Ps-CATH4 peptide

Ps-CATH4 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-TRGRWGRFKRRAGRFIRRNRWQIISTGLKLIG-OH

H-Thr-Arg-Gly-Arg-Trp-Gly-Arg-Phe-Lys-Arg-Arg-Ala-Gly-Arg-Phe-Ile-Arg-Arg-Asn-Arg-Trp-Gln-Ile-Ile-Ser-Thr-Gly-Leu-Lys-Leu-Ile-Gly-OH

32

95.2%

C₁₇₅H₂₉₁N₆₅O₃₈

3913.58

Synthetic

Powder

Cathelicidins are a class of gene-encoded immune effectors in vertebrate innate immune system. Ps-CATH4 is a novel cathelicidin which was found in Chinese soft-shell turtle (Pelodiscus sinensis). Ps-CATH4 was expressed in lung, spleen, liver, kidney, muscle, stomach and intestine. Ps-CATH4 showed the most potent and broad-spectrum antimicrobial activities against the tested pathogens, with the MICs mainly ranging from 2.34 μg/ml to 18.75 μg/ml. Ps-CATH6 showed relatively low antimicrobial activity. Ps-CATH4 and 6 showed relatively low cytotoxicity towards RAW264.7 cells, inducing 15.95% and 10.98% cell death, respectively. Ps-CATH4 showed more potent membrane permeabilization effect than Ps-CATH6, which is consistent with their different antimicrobial activities.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Shi N, Cai S, Gao J, et al. Roles of polymorphic cathelicidins in innate immunity of soft-shell turtle, Pelodiscus sinensis. Dev Comp Immunol. 2019

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.