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Product Name
PRRX1 Polyclonal Antibody
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Description
Polyclonal antibody to PRRX1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
PRRX1 antibody; AGOTC antibody; PHOX1 antibody; PMX1 antibody; PRX-1 antibody; PRX1 antibody; paired related homeobox 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PRRX1 (NP_073207.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
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Validations
Western blot - PRRX1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PRRX1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
Acts as a transcriptional regulator of muscle creatine kinase (MCK) and so has a role in the establishment of diverse mesodermal muscle types. The protein binds to an A/T-rich element in the muscle creatine enhancer (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"