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PRPF4 antibody

Cat.#: 114231

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Special Price 239.0 USD

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Product Information

  • Product Name
    PRPF4 antibody
  • Documents
  • Description
    PRPF4 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive WB detected in Raji cells. Observed molecular weight by Western-blot: 58kd
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    HPRP4 antibody; HPRP4P antibody; PRP4 antibody; PRP4 homolog antibody; Prp4p antibody; PRPF4 antibody; U4/U6 snRNP 60 kDa protein antibody; WD splicing factor Prp4 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PRPF4 recombinant protein (Accession Number: NM_004697). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:10-1:100

  • Validations

    Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:114231(PRPF4 antibody) at dilution of 1:500

    Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:114231(PRPF4 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114231(PRPF4 antibody) at dilution of 1:10 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114231(PRPF4 antibody) at dilution of 1:10 (under 10x lens)

  • Background
    PRPF4, also named as U4/U6 small nuclear ribonucleoprotein Prp4, is a 522 amino acid protein, which contains seven WD repeats. PRPF4 localizes in the Nucleus speckle. PRPF4 participates in pre-mRNA splicing and is a part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.PRPF4-mediated phosphorylation of KLF13 plays a role in the regulation of CCL5 expression in T lymphocytes
  • References
    • Li M, Aliotta JM, Asara JM. Intercellular transfer of proteins as identified by stable isotope labeling of amino acids in cell culture. The Journal of biological chemistry. 285(9):6285-97. 2010.
    • Tanackovic G, Ransijn A, Thibault P. PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa. Human molecular genetics. 20(11):2116-30. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"