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Product Name
PRPF31 Polyclonal Antibody
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Description
Polyclonal antibody to PRPF31
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
PRPF31 antibody; NY-BR-99 antibody; PRP31 antibody; RP11 antibody; SNRNP61 antibody; pre-mRNA processing factor 31 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRPF31 (NP_056444.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - PRPF31 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PRPF31 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - PRPF31 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using PRPF31 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PRPF31 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF31 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PRPF31 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using PRPF31 antibody at dilution of 1:100 (40x lens).
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Background
Involved in pre-mRNA splicing as component of the spliceosome. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"