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Human TGFA / TGF-alpha recombinant protein

Cat.#: 501636

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Product Information

  • Product Name
    Human TGFA / TGF-alpha recombinant protein
  • Documents
  • Description
    TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
  • Protein name
    Protransforming growth factor alpha
  • Protein short names
    TFGA; WA-1; TGFA; WA1; WAVED 1; TRANSFORMING GROWTH FACTOR ALPHA; PROTRANSFORMING GROWTH FACTOR ALPHA; TRANSFORMING GROWTH FACTOR; TGF-ALPHA; ALPHA
  • Uniprot ID
    P01135
  • Gene Name
    TGFA
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human TGFa (P01135-1) (Val40-Ala89) was expressed and purified.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions.
  • Purity
    > 90 % as determined by SDS-PAGE
  • Activity
    1. Measured by its binding ability in a functional ELISA. Immobilized human TGFa (cat:501636) at 10 μg/ml (100 μl/well) can bind human EGFR-Fc (Cat:504095). The EC50 of human EGFR-Fc (Cat:504095) is 20-60 ng/ml.
    2. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
    The ED50 for this effect is 0.1-0.5ng/mL.
  • Validations

    Human TGFA / TGF-alpha Protein SDS-PAGE

    Human TGFA / TGF-alpha Protein SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"