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Product Name
Human PRC1 (His Tag) recombinant protein
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Description
PRC1 (protein regulator of cytokinesis 1) is a key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. It is essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. PRC1 is required for KIF14 localization to the central spindle and midbody. It is also required to recruit PLK1 to the spindle. PRC1 stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. It is a homodimer and interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1. The interaction with RACGAP1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. PRC1 also interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. It interacts with KIF14, IF20A and PLK1.
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Protein name
Protein regulator of cytokinesis 1, isoform CRA_e
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Protein short names
PROTEIN REGULATING CYTOKINESIS 1; ANAPHASE SPINDLE ELONGATION 1 HOMOLOG; D7ERTD348E; ASE1
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Uniprot ID
O43663
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Gene Name
PRC1; hCG_32579
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human PRC1 isoform 1 (NP_003972.1) (Met 1-Ser 620) was expressed, with a polyhistidine tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human PRC1 consists of 639 amino acids and predicts a molecular mass of 74 kDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
Human PRC1 Protein (His Tag) SDS-PAGE
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