Human MAGOH (His Tag) recombinant protein

Human MAGOH (His Tag) recombinant protein

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Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator WIBG/PYM leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.

Protein mago nashi homolog

MAGO-NASHI HOMOLOG; PROLIFERATION-ASSOCIATED (DROSOPHILA); MAGO-M; MAGOHA; MAGOH1; MOS2; PROTEIN MAGO NASHI HOMOLOG; MAGOH; RP23-40G2.2; RP5-1024G6.4; MOS2

P61326

MAGOH; MAGOHA

E. coli

A DNA sequence encoding the human MAGOH (NP_002361.1) (Met1-Ile146) was expressed with a polyhistide tag at the C-terminus.

Human

The recombinant human MAGOH consists of 152 amino acids and predicts a molecular mass of 18 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE