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MAGOH antibody

Cat.#: 112455

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Product Information

  • Product Name
    MAGOH antibody
  • Documents
  • Description
    MAGOH Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive WB detected in K-562 cells, HeLa cells, HL-60 cells, human brain tissue, Raji cells. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 17 kDa
  • Tested applications
    ELISA, IHC, IP, WB
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    MAGOH antibody; MAGOHA antibody; Protein mago nashi homolog antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MAGOH recombinant protein (Accession Number: NM_002370). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112455(MAGOH Antibody) at dilution of 1:1000

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112455(MAGOH Antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112455(MAGOH antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112455(MAGOH antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-MAGOH (IP:Catalog No:112455, 3ug; Detection:Catalog No:112455 1:500) with K-562 cells lysate 2400ug.

    IP Result of anti-MAGOH (IP:Catalog No:112455, 3ug; Detection:Catalog No:112455 1:500) with K-562 cells lysate 2400ug.

  • Background
    MAGOH, belonging to the mago nashi family, is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and a core EJC component Y14.
  • References
    • Silver DL, Watkins-Chow DE, Schreck KC. The exon junction complex component Magoh controls brain size by regulating neural stem cell division. Nature neuroscience. 13(5):551-8. 2010.
    • Zhao W, Zhou F, Zhou X. Mago, a vertebrate homolog of Drosophila Mago nashi protein, is a component of the chromatoid body in the cytoplasm of the postmeiotic spermatid. Journal of experimental zoology. Part B, Molecular and developmental evolution. 314(3):232-41. 2010.
    • McMahon JJ, Shi L, Silver DL. Generation of a Magoh conditional allele in mice. Genesis (New York, N.Y. : 2000). 52(8):752-8. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"