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Product Name
PRL Polyclonal Antibody
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Description
Polyclonal antibody to PRL
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
PRL antibody; GHA1 antibody; prolactin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-227 of human PRL (NP_001157030.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - PRL Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PRL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - PRL Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using PRL antibody .
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Background
Prolactin acts primarily on the mammary gland by promoting lactation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"