Cart 2

PRKAR2A antibody

Cat.#: 114197

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    PRKAR2A antibody
  • Documents
  • Description
    PRKAR2A Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in K-562 cells, HeLa cells, human testis tissue, MCF7 cells, mouse testis tissue, PC-3 cells. Positive IF detected in HeLa cells, HepG2 cells, MCF-7 cells. Observed molecular weight by Western-blot: 46-52 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    PKR2 antibody; PRKAR2 antibody; PRKAR2A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PRKAR2A recombinant protein (Accession Number: NM_001321983). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114197(PRKAR2A antibody) at dilution of 1:1000

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114197(PRKAR2A antibody) at dilution of 1:1000

    IP result of anti-PRKAR2A(Catalog No:114197 for IP and Detection).

    IP result of anti-PRKAR2A(Catalog No:114197 for IP and Detection).

    Immunofluorescent analysis of HeLa cells using Catalog No:114197(PRKAR2A Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:114197(PRKAR2A Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"