PREB antibody

Cat.#: 114180

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Product Information

  • Product Name
    PREB antibody
  • Documents
  • Description
    PREB Rabbit Polyclonal antibody. Positive WB detected in SKOV-3 cells, 3T3-L1 cells, HepG2 cells, HT-1080 cells. Positive IHC detected in human prostate cancer tissue, human ovary tumor tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 45 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    PREB antibody; SEC12 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PREB recombinant protein (Accession Number: NM_013388). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    WB result of anti-PREB (Catalog No:114180, 1:250) with HepG2 and HT1080 by Dr. Dzmitry Batrakou.

    WB result of anti-PREB (Catalog No:114180, 1:250) with HepG2 and HT1080 by Dr. Dzmitry Batrakou.

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114180(PREB antibody) at dilution of 1:600

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114180(PREB antibody) at dilution of 1:600

    Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:114180(PREB Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:114180(PREB Antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells using Catalog No:114180(PREB Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:114180(PREB Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"