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Product Name
PREB antibody
- Documents
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Description
PREB Rabbit Polyclonal antibody. Positive WB detected in SKOV-3 cells, 3T3-L1 cells, HepG2 cells, HT-1080 cells. Positive IHC detected in human prostate cancer tissue, human ovary tumor tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
PREB antibody; SEC12 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PREB recombinant protein (Accession Number: NM_013388). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
WB result of anti-PREB (Catalog No:114180, 1:250) with HepG2 and HT1080 by Dr. Dzmitry Batrakou.
SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114180(PREB antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:114180(PREB Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114180(PREB Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"