Pre-S2 (1-26) peptide

Pre-S2 (1-26) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

MQWNSTAFHQTLQDPRVRGLYLPAGG

H-Met-Gln-Trp-Asn-Ser-Thr-Ala-Phe-His-Gln-Thr-Leu-Gln-Asp-Pro-Arg-Val-Arg-Gly-Leu-Tyr-Leu-Pro-Ala-Gly-Gly-OH

26

97.8%

C₁₃₁H₁₉₉N₃₉O₃₇S

2944.33

Synthetic

Powder

This synthetic peptide comprises the N-terminal portion of a 55-residue sequence encoded by the pre-S2 region of hepatitis B virus (HBV) DNA. It represents an immunodominant epitope, and the immunogenicity can be enhanced by covalently linking the peptide to liposomes. Antibodies elicited against this determinant recognize envelope epitopes common to several subtypes of HBV and HBsAg particles. Because these antibodies show virusneutralizing activity in vivo, pre-S (120-145) has the potential as

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• A.R.Neurath et al., Science, 224, 392 (1984)
• A.R.Neurath et al., J. Virol. Meth., 12, 185 (1985)
• A.R.Neurath et al., Nature, 315, 154 (1985)
• A.R.Neurath et al., Vaccine, 4, 35 (1986)
• A.R.Neurath et al., Synthetic Peptides Approaches to Biological Problems, UCLA Symposia on Molecular and Cellular Biology, New Series, Vol. 86, p. 143, Alan R.Liss, New York, (1989)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.