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Product Name
PRDX4 antibody
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Description
PRDX4 Mouse Monoclonal antibody. Positive IHC detected in human colon tissue, human pancreas cancer tissue, human prostate cancer tissue. Positive WB detected in K-562 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 31 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
Antioxidant enzyme AOE372 antibody; AOE37 2 antibody; peroxiredoxin 4 antibody; Peroxiredoxin IV antibody; PRDX4 antibody; PRX 4 antibody; Prx IV antibody; Thioredoxin peroxidase AO372 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of PRDX4 recombinant protein (Accession Number: NM_006406). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107482(PRDX4 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:107482(PRDX4 Antibody) at dilution of 1:50(unde10x lens)
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:107482(PRDX4 Antibody) at dilution of 1:50(under 40x lens)
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Background
PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).
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