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Product Name
PRDM10 antibody
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Description
PRDM10 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in HEK-293 cells, mouse testis tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 120-150 kDa
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Tested applications
ELISA, IP, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
KIAA1231 antibody; PFM7 antibody; PR domain containing 10 antibody; PRDM10 antibody; TRIS antibody; Tristanin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRDM10 recombinant protein (Accession Number: NM_199437). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114172(PRDM10 antibody) at dilution of 1:1000
IP Result of anti-PRDM10 (IP:Catalog No:114172, 5ug; Detection:Catalog No:114172 1:500) with mouse testis tissue lysate 8000ug.
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Background
PRDM10 is a member of PRDM family, which contains a PR(PRDI-BF1 and RIZ homology) domain, and PRDM is also a subgroup of PR/SET transcription factor family for its PR domain is similar to the catalytic motif of the SET domain of histone methyltransferase(HMTase). Thus PRDM is suggested involve in the regulation of genome expression and chromatin remodeling. PRDM10 shares characteristics with the PR/SET faimly and clusters of zinc fingers DNA binding motifs. It's postualated PRDM10 has an essential role in regulating gene expression and tissue differentiation and a gene repressor. It may play a role in the pathogenesis of gangliosidosis.
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