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Product Name
PRC1 antibody
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Description
PRC1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HEK-293 cells, HepG2 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ASE1 antibody; PRC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRC1 recombinant protein (Accession Number: NM_003981). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114169(PRC1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114169(PRC1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114169(PRC1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PRC1 (IP:Catalog No:114169, 4ug; Detection:Catalog No:114169 1:300) with HEK-293 cells lysate 1480ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:114169(PRC1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.
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