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Product Name
PRAS40 antibody
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Description
PRAS40 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in MCF-7 cells, HeLa cells. Observed molecular weight by Western-blot: 40 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
AKT1S1 antibody; AKTS1 antibody; Lobe antibody; PRAS40 antibody; Proline rich AKT1 substrate 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRAS40 recombinant protein (Accession Number: NM_001278160). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:50-1:500
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:114167(PRAS40 Antibody) at dilution of 1:1000
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:114167(PRAS40 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
AKT1S1 represents AKT1 substrate 1 (proline-rich)
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