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Product Name
PPT2 Polyclonal Antibody
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Description
Polyclonal antibody to PPT2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
PPT2 antibody; C6orf8 antibody; G14 antibody; PPT-2 antibody; lysosomal thioesterase PPT2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-302 of human PPT2 (NP_005146.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - PPT2 Polyclonal Antibody
Western blot analysis of extracts of mouse liver, using PPT2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - PPT2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using PPT2 antibody at dilution of 1:100 (40x lens).
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Background
Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S-thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"