PPP4C antibody

PPP4C antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

PPP4C Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, Jurkat cells, mouse kidney tissue. Positive IP detected in mouse kidney tissue. Observed molecular weight by Western-blot: 35 kDa

ELISA, WB, IP

Human,Mouse,Rat; other species not tested.

PP X antibody; PP4 antibody; PP4C antibody; PPH3 antibody; PPP4 antibody; PPP4C antibody; PPX antibody; Protein phosphatase X antibody

Rabbit IgG

This antibody was obtained by immunization of PPP4C recombinant protein (Accession Number: NM_002720). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine/threonine protein phosphatases (PP) are intimately involved in this process. Serine/threonine protein phosphatase X (PPX; also called protein phosphatase 4 (PP4)) are specifically associated with nuclear factor-kappa B (NF-kappa B) p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Overexpression of PPX stimulated the DNA-binding activity of c-Rel and activated NF-kappa B-mediated transcription. Although the mechanism by which PPX activates Rel/NF-kappa B-mediated transcription is unclear, PPX appeared to act on Rel/NF-kappa B proteins directly through augmentation of c-Rel activity. It is also possible that PPX may dephosphorylate and subsequently activate other c-Rel-associated transcription factors or other kinases regulating I kappaB (e.g. I kappa B kinases or MEKK1). These data suggest that PPX is an activator, but not an inhibitor, of c-Rel/NF-kappa B, which is in contrast to other protein phosphatases.

• Chen L, Dong W, Zou T. Protein phosphatase 4 negatively regulates LPS cascade by inhibiting ubiquitination of TRAF6. FEBS letters. 582(19):2843-9. 2008.